The expression of two-component system response regulator in multidrug-resistant Mycobacterium tuberculosis
10.3760/cma.j.issn.1009-9158.2011.09.007
- VernacularTitle:耐多药结核分枝杆菌双组份系统反应调节子表达的研究
- Author:
Lei ZHOU
;
Yueyun MA
;
Jiayun LIU
;
Fang HUANG
;
Mingquan SU
;
Liu YANG
;
Xiaoke HAO
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Drug resistance,bacterial;
Polymerase chain reaction;
Signal transduction
- From:
Chinese Journal of Laboratory Medicine
2011;34(9):800-804
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo screen out the two-component system associated with drug resistance of Mycobacterium tuberculosis by detecting the differential expression of two-component system regulator genes between multidrug resistant Mycobacterium tuberculosis strains and drug sensitive strains. MethodsTotal RNA of MTB was extracted from cultured MTB during the logarithmic phase in the 7H9 brook medium, and then its purity was identified. Reverse transcription was further completed. The expressing levels of TCS response regulators were quantified using SYBR Green I qRT-PCR, which aimed at finding the differential expressions between multidrug resistant strains and sensitive strains. Finally, all of differentially expressed TCS were screened out under the stress of INH, SM and LFA. Results Compared with sensitive strains,multidrug resistant strains of Rv0491, Rv3133c, Rv3143 and Rv3246c were up-regulated 1. 03, 7.11,3.48and 1.37 folds, respectively (t/t' =5. 623, -4. 196, -3. 559 and -3. 016, respectively, P ＜0. 01 ). The expressing level of other regulators had no statistical significance between muhidrug resistant strains and drug sensitive strains. Under the antibiotic pressure, the expression of Rv1027c, Rv3246c and Rv3143 showed significant changes compared with no antibiotic group. ConclusionRv3246c and Rv3143 may be associated with MTB drug resistance and the differentially expressed genes in multi-drug resistant strains may be used as potential drug targets against drug resistant tuberculosis.
