Effects of fluid shearing force on type Ⅱ collagen synthesis by human chondrocytes in vitro

VernacularTitle:流体剪切力影响人关节软骨细胞合成Ⅱ型胶原的研究
Author: Hua WANG ; Shiqing LIU ; Liaobin CHEN ; Biao CHEN
Publication Type:Journal Article
Keywords: Shearing force; Chondrocytes; Type Ⅱ collagen
From: Chinese Journal of Physical Medicine and Rehabilitation 2011;33(7):505-508
CountryChina
Language:Chinese
Abstract: Objective To detect type Ⅱ collagen synthesis by cultured human chondrocytes under the influence of different intermittent fluid shearing forces.Methods Second passage human monolayer chondrocytes were divided into low-speed (20 rpm/min), mid-speed (40 rpm/min) and high-speed (60 rpm/min) groups according to the rotation speed of the rocking bed. The expression of type Ⅱ collagen was analyzed using immunohistochemistry and RT-PCRs.Results The absorbance of the mid-speed and high-speed groups was significantly greater than that of the low-speed group and a control group. The average optical density of type Ⅱ collagen by RT-PCR was significantly higher in all three sheared groups than in the control group.Conclusions Intermittent shearing can enhance cellular proliferation and the metabolism of human chondrocytes in vitro and promote the expression of type Ⅱ collagen.