The research of that Shikonin effects on VEGF production in IL-17-stimulated HaCaT cells
10.3760/cma.j.issn.0254-5101.2011.08.003
- VernacularTitle:紫草素对IL-17刺激HaCaT细胞分泌VEGF影响的研究
- Author:
Min HANG
;
Long GENG
;
Hongwei REN
;
Huiming QU
;
Xue WANG
;
Yongzhi JI
;
Zhongxiang WEI
;
Hongbo ZHOU
- Publication Type:Journal Article
- Keywords:
Psoriasis;
HaCaT cell;
IL-17;
VEGF;
Shikonin
- From:
Chinese Journal of Microbiology and Immunology
2011;31(8):685-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. MethodsWe examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). ResultsThe expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P＜0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P＜0. 001 ). The cell viability of different drug treatment groups have no significant difference( P＞0.05 ). ConclusionWe show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatantThen shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.
