Preliminary study on the biological functions of interferon-λ in human esophageal carcinoma cells

VernacularTitle:IFN-λ在人食管癌细胞系中诱导的生物学活性的初步研究
Author: Xin ZHAO ; Danna ZHAO ; Jie HU ; Zhengmei YANG ; Youquan BU ; Lin WEI ; Quanhai LI ; Yixin QI
Publication Type:Journal Article
Keywords: IFN-λ; Antiviral activity; Antiproliferative activity; Immunoregulation
From: Chinese Journal of Microbiology and Immunology 2011;31(8):693-696
CountryChina
Language:Chinese
Abstract: Objective To investigate the biological function of IFN-λ in 7 human esophageal carcinoma cells. MethodsThe gene expression of IL-28α, IL-10β and antiviral molecule was examined with PCR. The MHC molecules expression and the profiles of cell cycle were analyzed with flow cytometer. Cell proliferation was evaluated with MTT assay. ResultsAll of esophageal carcinoma cells express the gene of II-28α and IL-10β. IFN-λ induced or augmented the gene expression of antiviral molecules, 2′5′-OAS and MxA. IFN-λ enhanced the MHC class Ⅰ molecule expression. IFN-λ inhibited the growth of esophageal carcinoma cells through the regulation of cell cycle distribution. ConclusionEsophageal carcinoma cells express the IFN-λ receptor complex. IFN-λ has the antiviral, anti-proliferative and immunoregulation activity.