Study on the antibiotic resistance, homology of imipenem-resistant Pseudomonas aeruginosa strains isolated from older
10.3760/cma.j.issn.0254-5101.2011.08.012
- VernacularTitle:老年人亚胺培南耐药铜绿假单胞菌耐药性研究
- Author:
Zhen XIAO
;
Fanghua FAN
;
Jie ZHU
;
Xiaomei YU
;
Huanqin ZHOU
;
Yunsong YU
- Publication Type:Journal Article
- Keywords:
Imipenem;
Pseudomonas aeruginosa;
Metallo-β-lactamase;
VIM-2;
Integron
- From:
Chinese Journal of Microbiology and Immunology
2011;31(8):718-723
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the antibiotic resistance, homology of imipenem-resistant Pseudomonas aeruginosa strains isolated from older in Zhejiang Hospital and the carbapenemases determinants of imipenem-resistant strains. MethodsTwo hundred and sixty-two strains of Pseudomonas aeruginosa were isolated through May 2006 to May 2009 from older in Zhejiang Hospitals. K-B method was used to determine the 16 antimicrobial agents resistance of these 262 strains. The MICs of strains to 14 antimicrobial agents were determined by agar dilution and E test method. The coding sequence of Metallo-β-lactamases (MBL) were amplified, PCR products were purified, cloned and sequenced. The homology of these isolates was analyzed by pulse-field gel electrophoresis(PFGE). ResultsOne hundred and four strains of imipenem-resistant Pseudomonas aeruginosa were screened from 262 strains. The resistant rates of 104 isolates of Pseudomonas aeruginosa to ampicillin/sulbactam and cefoperazone/sulbactam were 78.9％ and 35.9％ ; polymyxin E had a minimal resistance of 6.0％ ; minocycline had a resistance rate of 58.3％. The resistant rates to other antimicrobial agents were more than 70.0％. Twelve imipenem-resistant Pseudomonas aeruginosa strains contained MBL gene and two kinds of integron were detected from 10 of these 12 strains. Twelve strains of Pseudomonas aeruginosa belonged to 5 epidemic PFGE-clone. ConclusionAll of the imipenem-resistant Pseudomonas aeruginosa which had cause outbreaks in Zhejiang Hospital. MBL were not the most popular gene type. All of the MBL gene types were VIM-2. The blaVIM-2 gene cassettes located in diflerent class 1 integrons. The integrons dissemination was the most important style of strains spread.
