Prokaryotic expression of Neisseria gonorrhoeae NspA and its antigenicity

VernacularTitle:淋病奈瑟菌NspA原核表达及其抗原性研究
Author: Guocai LI ; Rushan XIE ; Guihua JIANG ; Litian ZHU ; Hongmei JIAO ; Xingyuan PAN ; Hongju CHEN ; Hua YAN ; Mingchun JI
Publication Type:Journal Article
Keywords: Neisseria gonorrhoeae; NspA; Antigenicity; Fast detection technique
From: Chinese Journal of Microbiology and Immunology 2012;32(5):395-398
CountryChina
Language:Chinese
Abstract: Objective To develop fast detection techniques for the diagnosis of gonococcal infections.Methods Prokaryotic expression vector for Neisserial surface protein A (NspA) was constructed using the NspA gene cloned by PCR.Mice were immunized with the renatured recombinant NspA (rrNspA)to prepare antibodies against NspA.Western blot and ELISA was used to analyze the binding of NspA antibodies to lysate of gonococcal cells or to intact gonococci.Results NspA antibodies that were prepared by the rrNspA expressed in E.coli could bind to rrNspA,the natural NspA existing in gonococcal cells,or intact gonococci.Conclusion RrNspA and its antibodies have potential value in developing fast diagnostic kits for gonococcal diseases.