Enhancing chondrogenic differentiation in precartilaginous stem cells with 620 nm red light

VernacularTitle:波长620nm红光促进前软骨干细胞向软骨细胞分化的实验研究
Author: Kunpeng LI ; Tao XU ; Yu DU ; Chen GONG ; Fei PENG ; Anmin CHEN ; Fengjin GUO
Publication Type:Journal Article
Keywords: Chondrocytes; Cell differentiation; Precartilaginous stem cells; Red light
From: Chinese Journal of Physical Medicine and Rehabilitation 2012;34(3):172-176
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of 620 nm red light on chondrogenic differentiation in rat precartilaginous stem cells (PSCs). Methods Rats' PSCs were isolated and purified using magnetically activated cell sorting and cultured in vitro.The PSCs were exposed once to 620 nm wavelength red light from a light-emitting diode (LED) with an irradiation energy of 0.5 J/cm2,1 J/cm2,2 J/cm2 or 4 J/cm2.Any effect was confirmed by Alcian blue staining,immunohistochemistry and observing histomorphological changes under a light microscope,as well as detection using a reverse transcription polymerase chain reaction (RT-PCR). Results After being induced for 14 d,the PSCs exhibited polygonal and round shapes. Alcian blue and type Ⅱ collagen immunohistoehemistry staining showed positive results,but the control group had no significant change.RT-PCR showed that the mRNA expression of Sox9 and type Ⅱ collagen increased significantly compared with the control group. Conclusion Low energy 620 nm red light can enhance chondrogenic differentiation in PSCs significantly.