Modulation of TNF on the expression of protease activated receptors in P815 mast mells
10.3760/cma.j.issn.0254-5101.2011.08.001
- VernacularTitle:肿瘤坏死因子调节的肥大细胞蛋白酶激活受体表达分析
- Author:
Huiyun ZHANG
;
Shufang ZHANG
;
Haiwei YANG
;
Wenjing MA
;
Shunlan WANG
;
Shaoheng HE
- Publication Type:Journal Article
- Keywords:
Mast cell;
TNF;
Protease activated receptor(PAR);
Flow cytometry;
Immunofluorescent microscopy
- From:
Chinese Journal of Microbiology and Immunology
2011;31(8):673-677
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential influence of TNF on the expression of protease activated receptor (PAR)-1,2,3 and 4 by using P815 mast cells. Methods After being challenged with various concentrations of TNF for 2 h, 6 h and 16 h, the P815 mast cells were treated with or without Triton X-100 and the PAR expressions were detected by flow cytometry and immunofluorescent microscopy. Results Compared with the corresponding controls, TNF concentration-dependently upregulated expressions of PAR-2 and PAR-4 both in Triton X-100-treated and the untreated groups, but had no significant effect on the expression of PAR-1 and PAR-3. Moreover, no significantly different expressions of TNF-induced PAR-1, 2, 3 were observed between Triton X-100-treated and the untreated groups, whereas Triton X-100-treated PAR-4 expressions were significantly enhanced by TNF compared with the Triton X-100-untreated ones. Conclusion TNF can up-regulate PAR-2, 4 expression of P815 mast cells but has little effect on the expression of PAR-1, 3 correspondingly. And Triton X-100 treatment had no significant effect on TNF-modulated expression of PARs in P815 mast cells.
