The research about preparation process and cell compatibility of MWCNT/n-HA/CS bone repair materials
10.3760/cma.j.issn.1001-2036.2011.04.013
- VernacularTitle:碳纳米管/羟基磷灰石/壳聚糖骨修复材料的制备及细胞相容性研究
- Author:
Wei QIAN
;
Aixi YU
;
Baiwen QI
;
Tao ZHANG
- Publication Type:Journal Article
- Keywords:
Carbon nanotubes;
Hydroxyapatite;
Chitasan;
Tissue engineering;
BMSCs
- From:
Chinese Journal of Microsurgery
2011;34(4):301-304
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the physicochemical properties and bicompatibility of carbon-nanotubes/hydroxypatite/chitosan scafflod for bone tissue engineering. MethodsMWCNT/n-HA/CS scaffolds wre generated by solution blending and freeze-drying technology.The morphology and composition of the scaffolds were analyzed by scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy, after this, the results of which mixed CNTS in scaffolds were evaluated. The effects of MWCNT/n-HA/CS scaffolds on adherence and proliferation of rabbit bone marrow stroma cells were assessed by scaffolds surface seeding methods, and using scanning electron microscopy, MTT assay to observe their adhesion and proliferation on scaffolds.Results MWCNT/n-HA/CS scaffolds showed abundant homogeneous pores with (87.26％) porosity. 66％ fracture strength of the scafflod was improved by MWCNT,and porosity decreased by 3％. Conclusion MWCNT/n-HA/CS scaffold can be prepared with solution blending and freeze drying process, which has fair poriness, good mechanical strength and tissue compatibility and can be applied as a bone graft material.
