Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing.
10.4041/kjod.2014.44.3.128
- Author:
Ebubekir TOY
1
;
Sengul YUKSEL
;
Firat OZTURK
;
Orhan Hakki KARATAS
;
Muhammet YALCIN
Author Information
1. Department of Orthodontics, Faculty of Dentistry, Inonu University, Malatya, Turkey. ebubekirtoy@gmail.com
- Publication Type:Original Article
- Keywords:
Genotoxicity;
Cytotoxicity;
Buccal epithelial cells;
Orthodontic composites;
Micronucleus test
- MeSH:
Cheek;
Epithelial Cells*;
Healthy Volunteers;
Humans;
Micronucleus Tests*;
Molar;
Mouth Mucosa;
Tooth
- From:The Korean Journal of Orthodontics
2014;44(3):128-135
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study evaluated the cytotoxicity and genotoxicity of fixed orthodontic treatment with three different light-cured orthodontic bonding composites by analyzing micronucleus (MN) formation in the buccal mucosa during a 6-month period. METHODS: Thirty healthy volunteers were selected from consecutive patients referred for orthodontic treatment. Equilibrium 2 brackets and molar tubes (Dentaurum) were bonded with three different light-cured orthodontic bonding composites-Transbond XT (3M Unitek), Kurasper F (Kuraray Europe), or GrenGloo (Ormco Corporation)- to all teeth in both arches. Exfoliated buccal epithelial cells were scraped from the middle part of the inner cheeks with sterile cement spatulas before treatment and at 1, 3, and 6 months after treatment. MNs and nuclear alterations, such as karyorrhexis (KR), karyolysis (KL), and binucleated cells (BNs), were scored under a light microscope. Repeated measure ANOVA was used to calculate statistical differences in degenerative nuclear abnormalities. RESULTS: MN rates did not significantly differ among different time points within the same cell type (p > 0.05). In contrast, the number of BNs in buccal epithelial cells significantly increased in all composite groups (p < 0.01, Transbond XT; p < 0.001, Kurasper F and GrenGloo). KL frequency significantly increased between the beginning and end of the study in the Kurasfer F (0.80 +/- 0.79 to 1.90 +/- 1.10; p < 0.05) and GrenGloo (1.30 +/- 1.06 to 2.40 +/- 1.08; p < 0.05) groups. CONCLUSIONS: After 6 months of fixed orthodontic treatment with different light-cured composites, morphological signs of cytotoxicity were observed but genotoxic effects were absent.
