bcl-2 antisense oligodexynucleotides enhances apoptosis in small-cell lung cancer cell line NCI-H69

VernacularTitle:bcl-2反义寡核苷酸对小细胞肺癌NCI-H69细胞凋亡的影响
Author: Yan ZHANG ; Yinghui BI ; Zhongxin ZHANG ; Weili JIA
Publication Type:Journal Article
Keywords: Genes/bcl-2; Carcinoma,small cell; Antisense oligodexynucleotides; Apoptosis
From: Cancer Research and Clinic 2011;23(8):515-517
CountryChina
Language:Chinese
Abstract: Objective To study the effect of bcl-2 antisense oligodexynucleotides on the apoptosis in human small-cell lung cancer cell line NCI-H69. Methods Cultured cells were divided into 4 groups: antisense oligodexynucleotides(ASODN), sense oligodexynucleotides (SODN), nonsense oligodexynucleotides (NSODN) and control.The different bcl-2 oligodexynucleotides was transfected into corresponding cells using oligofectamine.The expression of bcl-2 was examined by Western blot.The apoptosis rates were measured by flow cytometry (FCM).Results The bcl-2 expression in ASODN group was significantly inhibited compared to the control group, SODN and NSODN groups, but it was not obviously inhibited in SODN and NSODN groups.The apoptosis rate of ASODN group in different concentration was (9.97±1.54) %, (15.28±1.73) % and (21.41±1.85) % respectively, it was significantly higher than that of the control group (F = 7.19-15.48,q = 5.21-7.98, P ＜0.01). Conclusion The bcl-2 ASODN could enhance cell apoptosis rate in small-cell lung cancer by blocking bcl-2 gene effectively.