The study on gastrointestinal transit and changes of myenteric nerve plexus in acute necrotic pancreatitis rats complicated with hyperlipemia
10.3760/cma.j.issn.0254-1432.2011.07.008
- VernacularTitle:伴高脂血症急性坏死性胰腺炎大鼠胃肠传输与肠肌间神经丛形态改变研究
- Author:
Ting WU
;
Yuanchun YE
;
Fuchun ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute;
Hyperlipidemias;
Gastrointestinal metility;
Nitriergic neurons;
Cholinergic fibers;
Disease models,animal
- From:
Chinese Journal of Digestion
2011;31(7):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore association between the changes of myenteric nerve plexus and the delayed gastrointestinal transit in acute necrotic pancreatitis (ANP) rat complicated with hyperlipemia. Methods Atotal of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups (normal control group, ANP group, hyperlipemia (HL) control group and HL with ANP group). HL rat model was established by fed with high-fat diet for 4 weeks, and ANP rat model was induced by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. Gastrointestina1 transit distance was measured by ink gavage method. The histological changes of cholinergic and nitriergic nerves in myenteric plexus were observed by Karnovsky-Root method and NADPH histochemistry method. Results Pathological injuries were more severe in HL with ANP group than in ANP group (12.8±0.63 vs. 10.8±1.93,P<0.01), gastrointestinal transit was obviously delayed (transmission rate was 27%±5% vs. 38%±6%,P<0.01), the density of cholinergic neurons decreased (4.80±1.23 vs. 5.80±0.79, P<0.05), and the density of nitriergic neurons significantly increased (8.70±0.75 vs. 6.80±1.48, P<0.01). There was a linear regression between changes of cholinergic and nitrievgic nerves in myenteric nerve plexus and gastrointestinal transit (R2=0.531, P<0.01). Conclusion There was significant gastrointestinal motility disorder in the ANP rat complicated with hyperlipemia, which may be closely related with the changes of myenteric nerve plexus.
