Mechanism of dermal fibroblasts transdifferentiation into myofibroblasts induced by estrogen

VernacularTitle:雌激素诱导皮肤成纤维细胞向肌成纤维细胞表型转分化的机制
Author: Jie XIONG ; Qin LI ; Xiaobo CHEN ; Wenlin YU ; Biao CHENG
Publication Type:Journal Article
Keywords: Estrogen; Estrogen beta receptor; Fibroblast; Transdifferentiation; Kinase inhibitors
From: Chinese Journal of Medical Aesthetics and Cosmetology 2011;17(4):294-297
CountryChina
Language:Chinese
Abstract: Objective To explore the possible pathway and regulatory mechanism of dermal fibroblasts' transdifferentiation into myofibroblasts induced by estrogen. Methods Human dermal fibroblasts were divided into six groups (A: control; B: estrogen; C: estrogen + ICI-182780; D: estrogen + SB203580; E: estrogen + PD98059; F: estrogen + SP600125). The cells were collected for RNA extraction and the expression of α-SMA was detected by real time quantitative RT-PCR. Some cells were analyzed by single cell RT-PCR to detect positive expression percentage of α-SMA.Results The expression and positive rate of α-SMA in estrogen group were significantly increased (Group B vs. Group A, 7. 385±0. 246 vs 1. 367±0. 034, P＜0.01) and those in ICI-182780 group and SP600125 group were significantly inhibited (Groups C and F vs. Group B, 4. 619 ±0. 164,2. 409±0. 091 vs 7. 385±0. 246, P＜0. 05). Conclusions In the process of fibroblast transdifferentiation into myofibroblasts induced by estrogen, estrogen β receptor and JNK-MAPK signal transduction pathway may play an important role.