Research of the effect of persisters in Candida albicans biofilm on antifungal therapy using a Caenorhabditis elegans infection model
10.3760/cma.j.issn.0254-5101.2011.07.003
- VernacularTitle:用线虫模型研究白念珠菌生物膜中滞留菌对抗真菌治疗效果的影响
- Author:
Fen JIA
;
Qingguo QI
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Biofilm;
Persisters;
Antifungal;
Caenorhabditis elegans
- From:
Chinese Journal of Microbiology and Immunology
2011;31(7):588-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective To illuminate the effect of persisters on antifungal therapy by infecting Caenorhabditis elegans as a live-animal model with Candida albicans isolates of different persister levels, treating them with amphotericin B and comparing the survival rate. Methods Glp-4 (bn2ts); sek-1 (km4) worms were synchronized and grown to sterile to L4-stage, put on different Candida albicans strains lawns separately for 2 h. Dispensed 15-20 worms per well of the 96-well plate, and added serial dilutions of amphotericin B for each strain group. Wells filled without any amphotericin B were used as negative controls intra-group. Incubated the plate at 25C for 5 days, counted the survival rate of each well. Results Compared with negative controls, survival rate of drug wells in each group increased. In the same drug concentration, the increase for high-persister group was significantly lower than that for low-persister group (P＜0.01). Conclusion Caenorhabditis elegans provides a model for the study of persisters and antifungal pharmacodynamics.The drug tolerance of persisters may be a critical component responsible for antifungal drug failure and relapsing infections.
