The influence on the proliferation of rat B lymphocytes induced by β1-adrenoceptor autoantibodies
10.3760/cma.j.issn.0254-5101.2012.02.001
- VernacularTitle:β1-肾上腺素受体自身抗体对大鼠B淋巴细胞增殖的影响
- Author:
Tingting Lü
;
Yunhui DU
;
Jin WANG
;
Xiaoyu LI
;
Rui WANG
;
Huirong LIU
- Publication Type:Journal Article
- Keywords:
β1-adrenoceptor;
Autoantibodies;
B lymphocytes;
Proliferation
- From:
Chinese Journal of Microbiology and Immunology
2012;32(2):97-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of autoantibodies against the second extracellular loop of β1-adrenoceptor ( β1-AA) on the proliferation ability of LPS-stimulated rat B lymphocytes.Methods Active immunization assay was used to obtain adequate IgGs in which β1-AA was positive; MACS (magnetic activated cell sorting) assay was used for gaining rat splenic B lymphocytes; CCK8 assay was used for detecting the influence of β1-AA to the proliferation abilities of silent and LPS-stimulated rat splenic B lymphocytes.Results β1-AA (0.1 μmol/L pIgGs ) promoted the proliferation of LPS-stimulated rat splenic B lymphocytes(A values:0.739±0.036 vs 0.533±0.032,P＜0.05),and the effect was likely to be concentration-dependent.And the effect could be blocked by β1-AR blocker or β2-AR blocker partially,and could be blocked by β1-AR blocker and β2-AR blocker completely; β1-AA had no proliferation effect on silent rat splenic B lymphocytes.Conclusion β1-AA could promote the proliferation of LPS-stimulated rat splenic B lymphocytes via β1-AR and β2-AR which were on the surface of B lymphocytes.Thus,it could provide new clues for complex pathologic mechanism of cardiovascular patients in which β1-AA is positive.
