The cross immune responses of the rhesus monkey induced by the E style Chlamydia trachomatis recombinant major outer membrane protein
10.3760/cma.j.issn.0254-5101.2012.02.014
- VernacularTitle:E型沙眼衣原体重组主要外膜蛋白诱导恒河猴的交叉免疫效应
- Author:
Yiju LEE
;
Weifeng YAO
;
Xiaofei ZHAN
;
Jing WANG
;
Caihong SHENG
;
Yanfei LI
;
Bin FENG
;
Yuanjun LIU
;
Manli QI
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Recombinant major outer membrane protein;
Vaccine;
Immune response;
Rhesus monkey
- From:
Chinese Journal of Microbiology and Immunology
2012;32(2):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test cross immune responses induced in rhesus monkeys immunized with the recombinant major outer membrane protein(rMOMP).Methods Six rhesus monkeys were divided into three groups:the group vaccinated with purified rMOMP and Freund's adjutants,the group vaccinated with Freund's adjutants only and the control group vaccinated with PBS.All of the rhesus monkeys vaccinated intramuscularly at 0,2,4 weeks.Two weeks after the last time,The IFN-γand Chlamydia-specific antibody titers in sera,which were determined by ELISA,lymphocyte proliferation assay were performed by MTT,and observ the delayed hypersensitivity and in vitro neutralization assays.Results The result of the monkeys immunized with rMOMP and Freund's adjuvant:the specific immune responses can be observed.The in vitro neutralization and lymphocyte proliferation assays were observed better in the same group.Conclusion After being vaccinated with rMOMP,the monkeys can develop strong and effective Chlamydia-specific cross immune responses.
