Differences of the regulation on the expression of mucin 1 ( MUC1 ) induced by adenovirus serotype 5 and serotype 7 infections in airway epithelial cells
10.3760/cma.j.issn.0254-5101.2012.02.016
- VernacularTitle:腺病毒5型和7型感染诱导呼吸道上皮细胞黏蛋白1表达的差异性研究
- Author:
Mengwen ZHANG
;
Shuyuan NI
;
Yusheng LI
- Publication Type:Journal Article
- Keywords:
Adenovirus serotype 5;
Adenovirus serotype 7;
Airway epithelial cells;
Mucin 1 ( MUC1 );
Inflammatory regulation
- From:
Chinese Journal of Microbiology and Immunology
2012;32(2):172-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism for the self-limitation of adenoviral infections in human airway,the different impacts of adenovirus serotype 5 ( Ad5 ) and serotype 7 ( Ad7 ) infections on mucin 1 ( MUC1 ) expression in airway epithelial cells were preliminarily investigated.Methods The Ad5 and the Ad7 infection models were established in A549 cell line.qRT-PCR was performed to determine the transcription of MUC1 mRNA,and the expression of MUC1 in A549 cells infected by Ad5 or Ad7 was by detected Western blot.Results An up-regulation of the MUC1 mRNA level were observed after Ad5 infection for 6 h(P＜0.05 ),and the protein expression level of MUC1 increased in a time-dependent manner in 48 hours of Ad5 infection,while similar response of MUC1 mRNA was absent in Ad7 infection (6 h),even after prolonged (20 h) treatment ( P ＞ 0.05 ).Conclusion This study reveals an up-regulation of MUC1 expression as one of the early immune response to Ad5 infection,which implies that MUC1 may function fully or partially as an anti-inflammatory factor in the self-limitation effect of Ad5 infection.However,type7 adenoviral infection,may introduce a mechanism otherwise,but through MUC1.
