A Study on Gene Frequency of Paraoxonase Gene 2 in Patients with Coronary Heart Disease.
- Author:
Tae Youn CHOI
1
;
You Kyoung LEE
;
Won Bae KIM
;
Dong Wha LEE
;
Min Su HYON
;
Se Woong SEO
;
Sung Koo KIM
;
Young Joo KWON
Author Information
1. Department of Clinical Pathology, College of Medicine, Soonchunhyang University, Seoul, Korea
- Publication Type:Original Article
- Keywords:
Paraoxonase gene;
Polymorphism;
Coronary heart disease
- MeSH:
Aryldialkylphosphatase*;
Cholesterol, HDL;
Codon;
Coronary Angiography;
Coronary Disease*;
DNA;
Gene Frequency*;
Genotype;
Humans;
Leukocytes
- From:Korean Journal of Clinical Pathology
1999;19(4):420-424
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recent studies have implicated paraoxonase (PON), an HDL-associated enzyme, in providing protection against LDL oxidation, thus affecting the risk of coronary heart disease in general population. Serum PON activity is affected by PON gene polymorphism. We investigated the correlation between the PON2 codon 311 polymorphism (Cys-->Ser) and coronary heart disease in Koreans. METHODS: The subjects consisted of 145 healthy controls and 72 patients with coronary heart disease who were diagnosed by coronary angiography in Soonchunhyang University Hospital. The polymorphism at 311 codon in the PON2 gene was assessed by PCR-RFLP (restriction fragment length polymorphism) in their blood leukocyte DNA. RESULTS: PON2 genotype frequencies among 145 healthy controls were 7.6% CC, 37.2% CS and 55.2% SS (C:S = 0.250:0.750). PON2 genotype frequencies among 72 patients with coronary heart disease were 4.2% CC, 41.7% CS and 54.1% SS (C:S = 0.262:0.738). There was no significant difference between the two groups in genotype frequency or the allele frequency. There was no correlation between the PON2 polymorphism and HDL cholesterol level. CONCLUSIONS: The polymorphism at codon 311 in the PON2 gene are not associated with coronary heart disease in Koreans.
