Effects of high matrix metalloproteinase 9 on the biological behaviors of rat dermal fibroblasts.
10.3760/cma.j.issn.1671-0282.2012.10.009
- VernacularTitle:基质金属蛋白酶9高表达对大鼠皮肤成纤维细胞生物学行为的影响
- Author:
Shengneng XUE
;
Juan LEI
;
Chuan YANG
;
Diaozhu LIN
;
Li YAN
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinase 9;
Fibroblast;
Biological behaviors;
Proliferation;
Cell viability;
Secretion;
Migration;
Diabetic foot
- From:
Chinese Journal of Emergency Medicine
2012;21(10):1106-1110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of high matrix metalloproteinase 9 (MMP 9 ) on the biological behaviors of fibroblasts,in order to clarify the possible mechanisms in the wound healing of diabetic foot.Methods Establish the cell model of skin fibroblast with high expression of MMP 9 by high glucose (22.0 mmol/L) and high homocysteine (100 μmol/L) co-culture.Control group was incubated with normal glucose (5.5 mmol/L).Realtime PCR,ELISA and gelatin zymography were used to detect the MMP 9 mRNA,protein expression and activity of MMP 9.Flow cytometry,CCK-8,ELISA assay,scratch test and transwell were used to detect cell proliferation,viability,collagen (hydroxyproline) secretion,horizontal migration and vertical migration of cells.Results Data were expressed as (x- ± s).Differences were considered significant if their P value was ＜ 0.05.Results The expression of MMP 9 mRNA,protein levels and the activity of MMP 9 in high MMP 9 group were 6.05 folds,4.12 folds and 1.58 folds higher than those in control group (P ＜ 0.01,respectively).The proportion of S phase cells,proliferation index,cell viability,collagen (hydroxyproline) secretion,6 h horizontal migration rate and the number of vertical migration cells in high MMP9 group decreased by 29.8 ％,18.1％,23.3 ％,68.7 ％,45.0 ％ and 21.4 ％ than those in control group (P ＜ 0.01,respectively ). Conclusions Fibroblast with high expression of MMP 9 have decreased proliferation,activity,collagen secretion and reduced migration,which suggests that MMP 9 may inhibit the biological behaviors of fibroblasts.
