Development of Homemade Indicator Cells for Platelet Antibody Test by Mixed Passive Hemagglutination.
- Author:
Tae Hee HAN
1
;
Bok Yeon HAN
;
Hyun Jin JUNG
;
Kyou Sup HAN
Author Information
1. Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea
- Publication Type:Original Article
- Keywords:
Platelet antibody;
Mixed passive hemagglutination (MPHA);
Indicator cell
- MeSH:
Blood Donors;
Blood Platelets*;
Diagnosis;
Fluorescent Antibody Technique;
Healthy Volunteers;
Hemagglutination*;
Humans;
Immunoglobulin G;
Japan;
Korea;
Male;
Tissue Donors
- From:Korean Journal of Clinical Pathology
1999;19(4):446-452
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Platelet antibody test has been used in the diagnosis and management of immunological platelet disorders and platelet crossmatch. Mixed passive hemagglutination (MPHA) test is a cost-effective, reproducible, easy to perform and convenient method. Anti-IgG coated indicator red cells used for MPHA test have not been made in Korea and those cells have been exclusively donated by Dr. Shibata in Japan. This study was designed to produce domestic indicator cells and to determine its acceptability by comparing to the results obtained with Dr. Shibata's indicator cells. We produced homemade indicator cells by coating human RhD-positive O RBC with human IgG anti-D (check cell) and then coated with rabbit anti-human IgG. METHODS: Sixty three sera from healthy male donors, 58 sera tested positive in platelet suspension immunofluorescence test (PSIFT), and 61 sera tested negative in PSIFT were evaluated by MPHA employing both homemade and Dr. Shibata's indicator cells. RESULTS: The concordance rate between PSIFT and homemade MPHA was 74%. Test results of MPHA with homemade indicator cell showed excellent correlation with Shibata's indicator cell (P=0.002). All of 63 sera from healthy volunteer male blood donors without history of transfusion were tested negative with homemade MPHA method. Test results of MPHA employing homemade indicator cells showed excellent correlation with those of PSIFT (P=2.67x10-8). CONCLUSIONS: Homemade indicator cells we developed in this study were able to replace Dr. Shibata's indicator cells for the MPHA test.
