Effent of toosendanin on the biological behavior of human hepatoma cell line HepG2
10.3760/cma.j.issn.1008-6706.2012.20.003
- VernacularTitle:川楝素对人肝癌细胞株HepG2生物学行为的影响
- Author:
Fei ZHAO
- Publication Type:Journal Article
- Keywords:
Toosendanin;
HepG2;
Biological markers
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;19(20):3045-3046
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of toosendanin on proliferation and apoptosis of human hepatoma cell line HepG2 cells,and to study the mechanism.MethodsHuman hepatocellular carcinoma HepG2 cells were randomly divided into control group(group A) does not add any medication,the observation group(group B) joined TSN 80mmol/ml,cultured cells for 24,48,72 hours.HepG2 cells proliferation inhibition rate was measured by MTT; Hepatocellular carcinoma HepG2 cell cycle and apoptosis rate was deteded by flow cytometry.ResultsTECAN determination revealed two groups of hepatocellular carcinoma cell proliferation had statictical significance ( x2 =5.33,P ＜0.05 ) ;flow cytometry revealed two groups S,G0/G1,M/G2 phase of the cell rate ( t =6.31,6.26,6.56,all P ＜0.05 ),apoptosis rate had significant difference ( x2 =6.15,P ＜ 0.05 ).ConclusionToosendanin could obviously inhibit the proliferation of HepG2 cells,and induce apoptosis of tumor cells.
