Expression of somatomedin-receptor in anoxic prostate epithelial cells
10.3760/cma.j.issn.1673-4904.2012.26.001
- VernacularTitle:缺氧条件下前列腺上皮细胞体外培养生长类激素受体的表达
- Author:
Wen SHEN
;
Yongbin ZHAO
;
Ping LI
;
Cheng HUANG
;
Fei GUO
;
Jun Lü
;
Weilie HU
- Publication Type:Journal Article
- Keywords:
Anoxia;
Somatomedins;
Cell cultured techniques;
Prostate epithelial cell
- From:
Chinese Journal of Postgraduates of Medicine
2012;35(26):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the different expression of somatomedin-receptor in cell membrane of prostate epithelial cells at anoxic or normoxic condition.Methods Human prostate epithelial cells line RWPE-1 were cultured in vitro.At 4,8,12,24,48 h after cells had been seeded,the gene and protein expression of epidermal growth factor receptor (EGFR),fibroblast growth factor receptor (FGFR),transforming growth factor β1 receptor (TGF- β 1R),insulin-like growth factor-1 receptor (IGF-1 R) and vascular endothelial growth factor receptor (VEGFR) in prostate epithelial cells were tested by RT-PCR and immunohistochem-istry methods,respectively.Results The expression of mRNA and protein of EGFR,FGFR,IGF-1R,TGF- β1R,VEGFR were significantly increased in anoxic and normoxic prostate epithelial cells (P ＜ 0.01 ).At different time point,the expression of mRNA and protein of EGFR,FGFR,IGF-1R,TGF- β1R,VEGFR significantly higher in anoxic than those in normoxic prostate epithelial cells (P＜ 0.01 )besides 4 h EGFR mRNA,12 h EGFR protein,4 h IGF-1R mRNA,4 and 8 h IGF-1R protein,4 and 8 h TGF-β 1R mRNA,4 and 8 h TGF-β 1R protein,4 h VEGFR mRNA (P ＞ 0.05).Conclusion Anoxic prostate epithelial cell can up-regulate the expression of somatomedin-receptor.
