Influence of MBL on the expression of Bcl-2 and p53 gene in thyroid cancer cell lines
10.3760/cma.j.issn.1008-6315.2012.10.010
- VernacularTitle:甘露糖结合凝集素对甲状腺癌细胞p53及Bcl-2蛋白表达的影响
- Author:
Ying HAN
;
Geling LIU
;
Huiqin ZHANG
;
Fang YU
;
Weijuan LI
;
Yifang LU
;
Yanping SHI
- Publication Type:Journal Article
- Keywords:
Thyroid neoplasm;
Mannose-binding lectin;
p53;
Bcl-2
- From:
Clinical Medicine of China
2012;28(10):1039-1041
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of recombinant human mannose-binding lectin (RhMBL)on the expression of Bcl-2 and p53 gene in thyroid cancer cell lines.Methods Two kinds of thyroid cancer cell lines( K1 cells and FTC-133 cells) were dealed with different concentrations of rhMBL( control group:0 mg/L;experimental group:1 mg/L),then total protein was extracted to detect the expression of Bcl-2 and p53 by Western Blot.Results The expression level of p53 and Bcl-2 were observed to be obviously down-regulated after incubation with RhMBL.( 1 ) K1 cells:There was significant difference on the expression of p53 and Bcl-2 between control group and experimental group [ p53:( 1.51 ± 0.29) vs (0.74 ±0.07),t =7.63 ; P =0.040;Bcl-2:( 1.20 ± 0.07 ) vs ( 0.59 ± 0.04 ),t =6.28,P =0.001 ] ; ( 2 ) FTC-133 cells:there was also significant difference on the expression of p53 and Bcl-2 between control group and experimental group [ p53:( 0.88 ±0.14) vs(0.35 ±0.08),t =3.29,P=0.003; Bcl-2:(1.07±0.11) vs (0.33 ±0.06),t =5.73,P=0.000 ].Conclusion Bcl-2 and p53 may play an important role on the induction of thyroid cancer cell apoptosis and inhibition its proliferation by RhMBL.
