Effect of platelet-rich plasma on cellular proliferation and intracytoplasm calcium ion concentration of osteoblasts and tenocytes during the process of tendon-bone healing
10.3760/cma.j.issn.1671-7600.2012.04.012
- VernacularTitle:腱骨愈合过程中富血小板血浆对细胞增殖与胞浆内钙离子浓度的影响
- Author:
Wenliang ZHAI
;
Nan WANG
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Platelet-rich plasma;
Osteoblasts;
Tendon-bone healing
- From:
Chinese Journal of Orthopaedic Trauma
2012;14(4):331-335
- CountryChina
- Language:Chinese
-
Abstract:
Objectlve To explore the effect of platelet-rich plasma (PRP) on cellular proliferation and intracytoplasm calcium ion concentration of osteoblasts and tenocytes during the process of tendon-bone healing. Methods We established a kind of indirect co-culture system using transwell chambers to co-culture osteoblasts and tenocytes.The cellular proliferation was carried out in following manners:single culture of osteoblasts versus single culture of osteoblasts plus PRP and co-culture of osteoblasts versus co-culture of osteoblasts plus PRP; single culture of tenocytes versus single culture of tenocytes plus PRP and co-culture of tenocytes versus co-culture of tenocytes plus PRP.The cellular proliferation rates were measured by CCK-8 test.The intracytoplasm calcium ion concentration of was measured by laser confocal microscopy and fluo-3/AM. Results The proliferation rates and fluorescence intensities in the co-culture groups without PRP were significantly lower than other groups ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the single culture groups without PRP were significantly higher than in the co-culture groups without PRP but significantly lower than in the groups with PRP ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the groups with PRP were significantly higher than other groups ( P ＜ 0.05) but there was no significant difference between groups of the same cells ( P ＞ 0.05).The intracytoplasm calcium ion concentrations were proportional to the proliferation rates. Conclusions During the process of tendon-bone healing,PRP has a potential not only to wipe off the depression effect of osteoblasts and tenocytes co-cultured indirectly but also to enhance the cellular proliferation rate to a higher level.At the same time the calcium ion concentrations will be elevated.
