Ethanol decreases radiosensitivity of human breast cancer MCF-7 cells
10.3760/cma.j.issn.0254-5098.2012.02.014
- VernacularTitle:乙醇降低人乳腺癌MCF-7细胞放射敏感性的研究
- Author:
Lin ZHAO
;
Yansu WANG
;
Hang REN
;
Jiaying XU
;
Yang JIAO
;
Saijun FAN
- Publication Type:Journal Article
- Keywords:
Ethanol;
Breast cancer;
Radiosensitivity;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(2):162-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ethanol on radiosensitivity of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were divided into four groups including control group,ethanol treatment group,X-ray exposed group,and ethanol combined with X-ray group.Clonogenic assay was used to determine cell survival.Flow cytometry was employed to analyze cell cycle progression.Annexin V-FITC kit was used to determine cell apoptosis induction.Results Ethanol(50 and 100 mmol/L,50 h)had no influence on MCF-7 cell growth( t =0.82,1.15,P ＞0.05 ).The radiosensitivity of MCF-7 cells was reduced when the cells were pretreated with 50 mmol/L ethanol (t =4.15,P ＜0.05)and 100 mmol/L ethanol ( t =10.28,P ＜ 0.05 ) for 2 h. Compared with irradiation with X-ray alone,ethanol treatment decreased G2/M phase arrest(t =7.18,P ＜0.05) and sub-G1population(an indicator of apoptosis induction) ( t =5.39,P ＜ 0.05).A decrease of advanced and early apoptosis in the cells pretreated with ethanol was also confirmed by Annexin V-FITC apoptosis assay( t =4.86,7.59,P ＜ 0.05 ).Conclusions Ethanol causes radioresistance in human breast cancer MCF-7 cells,where the decreases of radiation-induced G2/M phase arrest and apoptosis may be involved.
