Chromosome aberration and micronucleus formation in human peripheral blood lymphocytes induced by 12C heavy ions
10.3760/cma.j.issn.0254-5098.2012.02.017
- VernacularTitle:重离子12C诱发人淋巴细胞染色体畸变和微核的研究
- Author:
Jie DU
;
Zhidong WANG
;
Xueqing ZHANG
;
Chuangao WANG
;
Xuekun YAN
;
Zhongwen WANG
;
Xiao WANG
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Heavy ions;
Chromosomal aberrations;
Micronucleus;
Biological effects
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(2):176-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological effects of 12C heavy ions with different LETs,and to construct the dose-effect curves of chromosomal aberrations and micronucleus.Methods Human peripheral blood lymphocytes were irradiated with 0.5 - 5.0 Gy of 60Co γ-rays,29 and 148 keV/μm 12C with a dose rate of 0.5 Gy/min,respectively.The colchicine method and cytokinesis-blocking method were applied to measure chromosome aberrations and micronuclei formation,respectively. Results The chromosomal aberrations induced by 29 and 148 keV/μm of 12C ion had a linear dose response,while the dose-response of micronucleus induction followed to a linear-quadratic model.Chromosome aberration and micronucleus frequency increased with the LET of 12C ions. However, the micronucleus frequency increased slowly when the radiation doses exceeded 3.0 Gy..Chromosomal aberrations induced by 148 keV/μm 12C ions increased significantly when the time of colchicine treatment was prolonged.Conclusions Chromosome aberration and micronucleus induced by 12C ions increased with the LET,and the micronucleus frequency increased with dose less than 3 Gy.The biological effects of 12C ions were stronger than those of low LET irradiation of γ-rays.
