Molecular epidemiology study on group A Rotavirus in hospitalized children with diarrhea in Shanghai during 2006-2008
10.3760/cma.i.issn.1000-6680.2012.02.008
- VernacularTitle:2006年至2008年上海市住院腹泻患儿A组轮状病毒的分子流行病学调查
- Author:
Lijuan LU
;
Jin XU
;
Huaqing ZHONG
;
Liyun SU
;
Lingfeng CAO
;
Menghua XU
- Publication Type:Journal Article
- Keywords:
Diarrhea;
Rotavirus;
Genotype;
Child;
Epidemiology,molecular
- From:
Chinese Journal of Infectious Diseases
2012;30(2):90-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic characteristic of molecular epidemiology of group A Rotavirus (RV) by analyzing viral genotypes,disease seasonality,and the patients' age distribution,so that to provide theoretical basis for preyention and control of RV diarrhea in children.MethodsA total of 380 RV antigen positive samples were selected from 5176 stool specimens collected from ＜5 year-old patients with acute diarrhea who were admitted to Children's Hospital of Fudan University during January 2006 to December 2008. Multiplex nested reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the RV genotypes.ResultsDuring 2006-2008,the incidence of RV related diarrhea peaked from October to December and about 96.8％ of all RV episodes occurred in patients younger than 3 years old,The predominant genotype was G3 which accounted for 58.4％ (222/380),G9 was an emerging genotype with the prevalence rate as high as 10.8％ (41/380).G1 and G2 types were rarely found during the three years.Infections with both G3 and G9 were the major mixed genotype G infection. Genotype P [8] was predominant with the prevalence rates of 64.6％ (53/82) and 46.8％ (58/124) in 2006 and 2008,respectively,whereasgenotype P[4] was predominant in 2007 (38,5％,67/174).P[6] and P[9] were found as minor types.The major mixed genotype P infection were genotype P[4] and P[8]. The proportion of undetermined genotype G and genotype P strains tended to increasing during 2006-2008.Genotype P [8]G3 was the major RV strain (20.5％) in Shanghai during 2006-2008 and the other prevalent genotypes included P[4]G3 and P[m]G3.Conclusion The infection of group A RV in Shanghai presents some new molecular epidemiology characteristics during 2006-2008,such as switch of predominant genotypes and diversification of prevalent genotypes.
