Inhibition of NF-kB enhanced X-ray irradiation induced apoptosis in human non-Hodgkin lymphoma
10.3760/cma.j.issn.0254-5098.2012.02.019
- VernacularTitle:NF-kB抑制与X射线诱导的人非霍奇金淋巴瘤细胞凋亡的研究
- Author:
Qiao QIAO
;
Yuanjun JIANG
;
Guang LI
- Publication Type:Journal Article
- Keywords:
Lymphoma;
NF-kB;
X-rays;
Survivin;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(2):182-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NF-kB in radiation-induced apoptosis ot human non-Hodgkin lymphoma (NHL) cells and the mechanism involved.Methods Three human non-Hodgkin lymphoma cell lines,Namalwa,Ramos,and Raji cells were divided into control,IR and IR + QNZ ( 10nmol/L) groups,respectively.Annexin-V kit was used to determine cell apoptosis. Protein expression levels of Survivin,Bax,Bcl-2 and cleaved Caspase-3 were evaluated by Western blot.Survivin mRNA was quantified by real-time PCR.Results Inhibition of NF-kB by QNZ pretreatment significantly enhanced X-ray induced apoptosis in human NHL cells in a dose-dependent manner (t =2.93 - 12.52,P ＜ 0.05).At the same time,QNZ significantly reversed the expression levels of Survivin protein and mRNA that were upregulated by radiation(t =3.29 - 16.72,P ＜ 0.05).QNZ also increased the levels of Caspase-3 and proapoptotic protein Bax,but reduced anti-apoptotic protein Bcl-2 expression level and hence the ratio of Bcl-2/Bax.Conclusions Inhibition of NF-kB could enhance radiation-induced cell apoptosis in human NHL cells through down-regulating Survivin expression and decreasing Bcl-2/Bax ratio.
