Effects of Tongxinluo on cvtokine EPO mRNA of rats brain after ischemical reperfusion injury
10.3760/cma.j.issn.1673-4246.2012.06.011
- VernacularTitle:通心络对缺血再灌注损伤大鼠脑组织细胞因子EPO mRNA变化的影响
- Author:
Changjun LU
;
Junlei LU
;
Hongwei AN
;
Guocheng LIU
;
Zheyi ZHOU
;
Bingxin WEI
;
Bingxun LU
;
Lixin WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
EPO mRNA;
Tongxinluo
- From:
International Journal of Traditional Chinese Medicine
2012;34(6):516-518
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the expression of hemopoietin(EPO) mRNA on cerebral ischemia-reperfusion injury in rats brain tissue and the effect of Tongxinluo on it.Methods The model of rat (MCAO) were perfused with Tongxinluo,the changes of neural stem cell proliferation and differentiation related cell factors of EPO mRNA were detected after ischemia-reperfusion injury 3、5、7、14 d by means of reverse transcriptase polymerase chain reaction(RT-PCR).ResultsEPO mRNA of ischemia-reperfusion models showed expression in different period,the expression enhanced in the third day,reached the highest in the fifth day; the ischemia side EPO mRNA expression enhanced in the third day after give Tongxinluo,in the 5,7 and 14 day,PCR expression gray values were higher than the model group.ConclusionEPO mRNA expression enhanced after cerebral ischemia,this expression can be strengthened by Tongxinluo,and may further induce neural stem cell proliferation and differentiation.
