The effect of different illumination condition on drosophila circadian rhythm and 24 h sleep time
10.3760/cma.j.issn.1673-4246.2012.05.012
- VernacularTitle:不同光照条件对果蝇昼夜节律与睡眠时间的影响
- Author:
Yang JIN
;
Hongsheng MAN
;
Ji ZHANG
;
Tingli LI
- Publication Type:Journal Article
- Keywords:
Drosophila;
Circadian Rhythm;
Biological Clock;
LED
- From:
International Journal of Traditional Chinese Medicine
2012;34(5):422-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of illumination changes on the drosophila circadian rhythm and 24 h sleep time.Methods We collect the unmated fruit flies (a total of 128,single-sex) under CO2 anesthesia within 8 hours of eclosion.And put them into the group of normal light group,day and night reversed group,24 h light group and 24 h dark group with the simple random sampling method,male and female with 4 single-sex groups(n=32).Change the illumination conditions of the living environment of the fruit flies and use Drosophila Activity Monitoring System (DAMS) and Data Acquisition System (DAS),to observe and compare the changes of drosophila circadian rhythm and 24 h sleep time between the different illumination conditions.Results Compared with the normal light group,drosophila circadian rhythm of the day and night reversed group was significantly reversed,the number of fruit flies' activities of 24 h light group was increased,and circadian rhythm was decline,drosophila circadian rhythm of the 24 h dark group is still there,but frequent activity appears during the day,and the 19 : 00 activity peak disappears.Conclusion The drosophila circadian rhythm and 24 h sleep time changes with illumination conditions change.
