Application of Polymerase chain reaction technique and Immuno-chromatography assay to detect antibody against M. tuberculosis in pleural fluid patients
- Author:
Nguyen Xuan Thanh
- Publication Type:Journal Article
- Keywords:
pleural fluid, tuberculosis,
- MeSH:
Polymerase Chain Reaction;
Diagnosis;
Tuberculosis
- From:Journal of Practical Medicine
2004;471(1):21-23
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
At the Hospital No 103, PCR technique was used for detecting M. Tuberculosis immediately in pleural fluid of 39 patients with tuberculosis pleuresia and 10 patients with primary cancer pleuresia. By PCR reaction tuberculosis bacterial DNA was detected successfully in pleural fluid of 92,3% of paitents. The technique had got 63,8% of sensitivity and 100% of speccificity. By immunochromatography, IgG antibacterial antibody in pleural fluid was detected in 89,7% of patients. The combination of these 2 methods were necessary in giving high efficacy in definite diagnosis. The technique of PCR could be applied in various institution for earlier diagnosis with higher accuracy.
