TCL1 and THRA gene expression levels of peripheral blood mononuclear cell in patients with Sj(o)gren syndrome
10.3760/cma.j.issn.1007-7480.2012.04.002
- VernacularTitle:实时定量聚合酶链反应检测干燥综合征患者外周血单个核细胞TCL1和THRA基因表达的研究
- Author:
Yongjing CHENG
;
Zheng WANG
;
Fang WANG
;
Chunmei ZHANG
;
Shu WANG
;
Jian LI
;
Cibo HUANG
- Publication Type:Journal Article
- Keywords:
Sj(o)gren syndrome;
Gene;
Peripheral blood mononuclear cell
- From:
Chinese Journal of Rheumatology
2012;16(4):220-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify novel markers such as THRA or TCL1 in Sj(o)gren's syndrome (SS) patients to discriminate them from hcalthy volunteers.Methods Experimental group (n=40) and healthy volunteer group (n=40) were recruited based on strict inclusion and exclusion criteria.Peripheral blood samples (9 ml) were collected and peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation.RNA was extracted using Trizol reagent,and the expression of THRA,TCL1 in PBMCs was detected by real-time PCR.And the data were processed with Wilcoxon test and t test (P＜0.05).Results TCL1 and THRA expression level are higher in SS patients (2.5±4.7) than healthy volunteers (Z=-2.045,P＜0.05).The TCLI expression level (4.4±3.8) is higher in high lymphadenopathy activation index patients (2 to 3grade) than that in low lymphadenopathy activation index patients (grade 0 to 1 ) (t=-2.319,P＜0.05 ).Conclusion TCL1 expression level is higher in SS patients,and TCL1 expression level is related to the severity of lymphadenopathy,which provide a new platform of the study for the pathogenesis,disease severity evaluation,even dia-gnosis and treatment of SS.
