The effects of RNA interference on the different fragments of osteopontin expression in hepatocellular carcinoma
10.3760/cma.j.issn.1007-8118.2012.04.014
- VernacularTitle:RNA干扰对骨桥蛋白不同片段表达的影响
- Author:
Fan LIN
;
Weili GU
;
Shaofeng FAN
;
Kunping LI
;
Chunming LIN
;
Zhenghao PEI
- Publication Type:Journal Article
- Keywords:
Osteopontin segment;
Hepatacellular carcinoma;
RNA interference
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(4):288-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective Within human hepatoma cell lines,we aimed to investigate the effects of the down-regulation by RNAi on different fragments of osteopontin (OPN) in order to discover more effective and accurate sites for OPN.Methods Specific small interfering RNA of OPN (OPNi-1) were synthesized and transfected into human hepatoma cell line (HEP-G2).Fluorescent quantitative PCR and immunohistochemical methods were used to test*the OPN expression levels of mRNA and protein before and after RNAi.Results After transfection,the △CT value of the A fragment was greater than B and C fragments of OPN mRNA in HEP-G2.Before RNAi was added to HEP-G2 cells,the three fragments A,B,C had OPN mRNA CT values of 8.31±1.58,8.78±1.49,8.25±1.51 respectively.Once the RNAi were added,the CT values were measured 48h after for the fragments A,B,and C which were 12.14±1.43,10.22±1.97,10.48±1.88 (P＜0.05) respectively.The immunohis tochemical values of A,B,C were down from 6.44±1.67,5.43±2.05,5.45±2.52 to 2.84±1.52,4.43± 1.65,3.95± 1.43 respectively after interference.Conclusions RNAi can inhibit the expression of OPN gene selectively.siRNA targets different segments of OPN,which may have more effects on invasion and metastasis of liver cancer for a more important significance in science and health economics.
