Ultrastructural changes of genital tract Chlamydia trachomatis after treatment with azithromycin in vitro
10.3760/cma.j.issn.0412-4030.2012.04.002
- VernacularTitle:生殖道沙眼衣原体体外经不同浓度阿奇霉素作用后超微结构的变化
- Author:
Hongwei CAI
;
Juanhua LIU
;
Mukai CHEN
;
Hongmei LI
;
Renxin CAI
;
Qinfen ZHANG
;
Jiande HAN
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Azithromycin;
Microscopy,electron
- From:
Chinese Journal of Dermatology
2012;45(4):228-230
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the ultrastructural changes of Chlamydia trachomatis after treatment with azithromycin.Methods The Chlamydia trachomatis laboratory strain (D/UW-3/Cx) was cultured in McCoy cells with or without the presence of azithromycin of 0.0667,0.1340,0.1900,0.2680 and 0.3330 mg/L for 48 hours.The ultrastructural changes of host cells andChlamydia trachomatis were observed by transmission electron microscopy.ResultsAfter 48-hour culture,vesicles increased in number both inside and outside of the inclusion bodies with the rise in azithromycin concentration; there were abnormally large reticulate bodies,some of which experienced abnormal division and even necrosis or breakdown; the number of elementary bodies was decreased,while their size was enlarged,with a more wrinkled outer membrane.No inclusionbodieswereseenwhentheconcentrationofazithromycinwas0.333mg/L. Conclusions Azithromycin can induce an increment in the outer membrane of Chlamydia trachomatis,formation of vesicles,abnormal enlargement or breakdown of reticulate bodies,and a decrease in elementary bodies.
