The research of compound matrine injection inducing human hepatoma SMMC-7221 cell to apoptosis
10.3760/cma.j.issn.1673-4246.2012.03.008
- VernacularTitle:复方苦参注射液诱导人肝癌SMMC-7221细胞凋亡的研究
- Author:
Na LEI
;
Bo ZHANG
;
Yingxia PEI
;
Xin QI
;
Jinhua WANG
;
Yun ZHANG
;
Rui LIU
;
Jie LI
;
Hongsheng LIN
- Publication Type:Journal Article
- Keywords:
Compound matrine injection;
smmc-7721 cells;
Cell apoptosis
- From:
International Journal of Traditional Chinese Medicine
2012;34(3):217-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the compound matrine injection on human hepatoma smmc-7721 cells' proliferation.Methods Human hepatoma smmc-7721 cells were sterilely cultured.Assayed the effects of compound matrine injection on smmc-7721 cells by MTT.The concentrations of the compound matrine injection were 3.34,2.38,1.43 and 0.48 mg/ml; Used the double staining method of annexin V-fluorescein isothiocyanate(Annexin V-FITC)/propidium iodide (propidium iodide,PI) to measure the apoptosis of hepatoma cell.The concentrations of the compound matrine injection were 0.86,1.72,and 3.43 mg/ml.Results The apoptosis rates of compound matrine injection with different concentration of 3.34,2.38,1.43 and 0.48 mg/ml on liver cancer cell SMMC-7721 were51.03％、53.67％、49.83％ and 0.03％ respectively after 24 hours; and 67.14％、65.35％、62.25％ and 0.05％ respectively after 48 hours;and 74.16％、68.77％、66.04％ and 26.02％respectively after 72 hours.The apoptosis rates of compound matrine injection with different concentration of 0.86,1.72 and 3.43 mg/ml on liver cancer cell SMMC-7721 were(2.86±0.35)％,(15.16± 1.15)％and(13.17±0.40)％ respectively after 24 hours; and (8.57±0.44)％,(28.07±0.76)％ and (29.81±8.10)％respectively after 48 hours,which were significantly higher than the control group (1.05±0.09)％(P＜0.05).Conclusion The compound matrine injection has a significant inhibition on human hepatoma smmc-7721 cells' proliferation and the mechanism was its inducing cell apoptosis.
