Affection of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment
10.3760/cma.j.issn.1673-4246.2012.03.009
- VernacularTitle:紫丹饮含药血清在子宫内膜免疫微环境中对内膜容受性的调节
- Author:
Qian CHEN
;
Zhe JIN
;
Qiudan LU
;
Ying CAO
;
Nan LI
- Publication Type:Journal Article
- Keywords:
Zidan Decoction;
Endometrial receptivity;
Uterine Natural Killer cells;
Co-culture
- From:
International Journal of Traditional Chinese Medicine
2012;34(3):220-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment,and then to discuss if it's effect on endometrial receptivity through its controlling the expression of cell factors which related to endometrial receptivity.Methods Mice's endometrium tissue samples and uterine Natural Killer (UNK) cells were obtained from pregnant mice on gd 4.5 days and gd 12.5 days.After endometrial glandular epithelial cells,endometrial stromal cells and UNK cells were separated through primary culture,epithelial cells and endometrial stromal cells at rate of 1 ∶ 1 a certain concentration UNK were mixed together and highly purified-cultured.Different concentrations of Zidan decoction was acted on the culture solution.FBS,black and aspirin control group were setup for contrast.The expression of LIF and VEGF which influenced by Zidan decoction in the immunity environment was assayed by Realtime-PCR.Results The expression of LIF and VEGF was significantly increased in the immunity environment of uterine (in 10％ Zidan decoction group,10％ aspirin group and 10％ blank control group:LIF was 2.10±0.20,1.98±0.14,0.90±0.05 separately,and VEGF was 3.17±0.31,2.52±0.09,0.92±0.06respectively,P＜0.05).Conclusion Zidan decoction could improve endometrial receptivity by increasing the expression of cell factor which related to endometrial receptivity with the help ofUNK paracrine.
