Effect of RNA interference for OPN on MDA-MB-231 cells and the growth of transplanted tumor in nude mice
10.3760/cma.j.issn.1007-631X.2012.04.021
- VernacularTitle:Osteopontin RNAi对人乳腺癌细胞MDA-MB-231生物学行为的影响
- Author:
Li YANG
;
Ling WEI
;
Wei ZHAO
;
Xianrang SONG
;
Xingwu WANG
;
Gang ZHENG
;
Meizhu ZHENG
;
Wenshu ZUO
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Osteopontin;
Anoxia;
RNA interference
- From:
Chinese Journal of General Surgery
2012;27(4):322-325
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of OPN in human breast cancer cell line ( MDA-MB-231) by using small interfering RNA to specifically knockdown OPN expression. MethodsOPN ShRNA expression vector was stably transfected to MDA-MB-231 cell line.The expression of OPN mRNA and protein were analyzed using reverse transcription polymerase chain reaction (RT-PCR)and Western blot,respectively.The growth of MDA-MB-231 cells were observed by MTT.The effect of OPN siRNA on the transplanted tumor growth and tumor hypoxia were assessed in nude mice. ResultsThe expression level of OPN in MDA-MB-231 cells were significantly lower under hypoxia or normoxia(P ＜ 0.05 ).OPN silence with RNAi significantly inhibited the invasion ability and proliferation of MDA-MB-231 cell lines (P ＜ 0.01 ).Inhibition of OPN with RNAi significantly inhibited the growth ability of MDA-MB-231 cells in vivo(P ＜0.05).The tumor hypoxia significantly decreased(P ＜ 0.05). ConclusionsOPN silence with RNAi can effectively inhibit cell proliferation and tumor growth of MDA-MB-231 cells,and decrease the bypoxia level of MDA-MB-231 transplanted tumor in nude mice.
