Determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets by RP-HPLC
10.3760/cma.j.issn.1673-4246.2012.03.010
- VernacularTitle:生化片中异鼠李素-3-O-新橙皮糖苷含量的RP-HPLC测定
- Author:
Lianying LU
- Publication Type:Journal Article
- Keywords:
ShenghuaTablets;
Isorhamnetin-3-O-nehesperidine;
RP-HPLC
- From:
International Journal of Traditional Chinese Medicine
2012;34(3):224-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of Isorhamnetin3-O-nehesperidinein Shenghua Tablets.Methods The RP-HPLC system was consisted of the Luna-C18(4.6 mm×250 mm,5 μm) column,the mobile phase was acetonitrile -0.4％H3PO4 (10 ∶ 90),the flow rate was set at 0.8 ml/min,the column temperature was 30*℃,and the UV detector was 254 nm.Results The linear response range was 0.056～ 1.12 mg/ml (r=0.9997).The average recovery of mangiferin was 98.0％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets.
