Purification of bone marrow mesenchymal stem cells by a Colony-Forming system
10.3760/cma.j.issn.1008-1372.2012.03.005
- VernacularTitle:克隆筛选法体外纯化骨髓间充质干细胞
- Author:
Yali YANG
;
Tao SUN
;
Jin FU
;
Yucheng XIE
- Publication Type:Journal Article
- Keywords:
Cloning,molecular/MT;
Mesenchymal stem cells;
Bone marrow cells
- From:
Journal of Chinese Physician
2012;14(3):304-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify BMSCs by a simple Colony-Forming system and identify BMSCs in vitro.Methods BMSCs were planted at low-density ( 10/cm2 ) in the early stage of isolation,additional cells were scraped until a colony formed,secondly seeded those Colony-Forming cells at low-density.This process was repeat again when passaged cells formed another colony.This so called Colony-Forming selection was repeated several times until highly purified cells were obtained.Biological characters of BMSCs were observed.The surface antigens of BMSCs were identified by flow cytometry.The multipotentiality of BMSCs was assayed for differentiation into either osteoblasts or adipocytes.Results Homogeneous cells were obtained after BMSCs were passaged twice to thrice by Colony-Forming system.These BMSCs highly expressed positive surface antigens of BMSCs ( CD29 in 98.8％,CD90 in 98.4％ ) meanwhile seldom expressed negative surface antigens of BMSCs (CD31 in 2.6％,CD45 in 3％ ).BMSCs efficiently differentiated into osteoblasts and adipocytes.Conclusions Colony-Forming system is a simple and effective way to get highly purified BMSCs in a short time.
