Mice islet allograft tolerance induced by blockade of OX40/OX40L and CD40/CD154 costimulation pathway
10.3760/cma.j.issn.0254-1785.2012.03.011
- VernacularTitle:阻断OX40和CD40协同共刺激信号延长小鼠胰岛移植物存活时间
- Author:
Shuqiu CHEN
;
Ming CHEN
;
Bin XU
;
Quan REN
;
Yiduo WANG
;
Xuhui WANG
;
Xianchang LI
- Publication Type:Journal Article
- Keywords:
Mice;
Islets of langerhans transplantation;
Antigens,OX40;
Antigens,CD40;
Graft survival
- From:
Chinese Journal of Organ Transplantation
2012;33(3):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of blockade of OX40/OX40L costimulation pathway on mice islet allograft tolerance in CD40/CD154 costimulation pathway blockade mice.Methods C57BL/6 mice were induced into diabetes mellitus as recipients,and were transplanted with DBA/2 mice islets.The recipients were divided into four groups,(1) treated with IgG as controls,(2) anti-OX40L mAb,(3) anti-CD154,(4) combined treatment of anti-OX40L mAb and anti CD154mAb.The mean survival time (MST) of islet allograft was observed.The expression of OX40 in activated T cells of CD154 deficient mice was detected.Effector T cells were obtained from the spleen of CD154 deficient mice cultured with or without anti-OX40L mAb for 3 days.The proliferation of T cells was assayed.Results The MST in the control group,anti-OX40L mAb group,anti-CD154 mAb group and anti OX40L mAb + anti-CD154 mAb group was 19,22,48,and ＞150 days respectively (P ＜0.05).The OX40 expression was readily induced in the 66％ activated T effector cells.CD154 deficient T effector cells proliferation was inhibited by the addition of anti-OX40L mAb in the culture in a dose-dependent fashion.Conclusion The blockade of OX40/OX40L costimulation pathway can promote islet allograft tolerance in CD40/CD154 costimulation pathway blockade mice by inhibiting the proliferation of T cells.
