The role of Survivin in the apoptosis of PC-3 cells induced by Oridonin
10.3760/cma.j.issn.1008-1372.2012.03.003
- VernacularTitle:Survivin在冬凌草甲素介导下对人前列腺癌细胞PC-3凋亡的作用
- Author:
Jin LI
;
Luoyan YANG
;
Hongtao WU
- Publication Type:Journal Article
- Keywords:
Microtubule-associated proteins/ME;
RUBESCENSINE/PD;
Prostatic neoplasms/ZD/PA/ME;
Apoptosis/DE
- From:
Journal of Chinese Physician
2012;14(3):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis-inducing effect of Oridonin on PC-3 cells line and the role of Survivin in the process.Methods After PC-3 cells were incubated with different concentrations of Oridonin,cell viability was analyzed with MTT assay.The percentage of earlier apoptosis cell was analyzed by flow cytometry.The protein expression of Survivin in PC-3 cells were detected by Western blot and fluorescent quantitative PCR.Results Oridonin effectively inhibited the proliferation of PC-3 cells in a concentration-time dependent way.After PC-3 cells were treated with Oridonin ( 2.5,5,10,20,40 μmol/L)for 48 hours,the cytotoxicity index were 9.2％,25.3％,39.3％,77.2％,92.5％ and the IC50 of PC-3 cells was 10.29 μmol/L,respectively.Flow cytometry was used to detect the effect of different concentration of Oridonin (0,10,20,40 μmol/L) for 48 hours,the apoptotic rates of PC-3 cells were 4.8％,15.4％,19.5％,27.4％ ( P ＜ 0.05).Oridonin down-regulated Survivin protein in a concentration-dependent way in PC-3 cells.Conclusions Oridonin can induce the apoptosis of PC-3 cells by a concentration-dependent manner.Oridonin can induce the apoptosis of PC-3 cells by down-regulated Survivin protein.
