The effects of isoflurane anesthesia on the medium-and long-term cognitive function in mice at different months of ages
10.3760/cma.j.issn.0254-9026.2012.03.019
- VernacularTitle:异氟烷麻醉对不同月龄小鼠中远期认知功能的影响
- Author:
Zhen HUA
;
Yin ZHOU
;
Dongxin WANG
- Publication Type:Journal Article
- Keywords:
Anesthesia,inhalation;
Cognitive
- From:
Chinese Journal of Geriatrics
2012;31(3):243-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of isoflurane anesthesia on the medium- and longterm cognitive function in mice at different months of age. Methods Male C57BL/6J mice at ages of 4 month (n =45)and 20 month (n 45) were randomly assigned to control group,short- term anesthesia and long term anesthesia groups,respectively (n=15 per group).Inhalation anesthesia was applied with 3％ isoflurane for induction and 1.2 ％ isoflurane for maintain in 30 ％ oxygen,and control group received 30％ oxygen only for 60 min.Short- and long- term anesthesia group anesthesia was maintained for 30 min and 60 min,respectively.Cognitive function was determined by alternative behavior and water maze.Alternative behavior was assessed at 1 d before ancsthesia and 7,14 and 28 d after anesthesia,and water maze was assessed from 7 d to 8 d,9 d,10 d,11 d and 28 d after anesthesia. Results The correct rate of alternative behavior at age of 4 month in long-term anesthesia group [(58=6)％] was lower than relative control [(69±9)％] (t=4.26,P＜0.01) at 28 d.The number of arm entries at age of 20 month in short- term anesthesia group (31 ± 6) was increased than control(24±6) and long-term anesthesia group (24±8)(F=5.34,P＜0.01) at 14 d after anesthesia,while the number in long-term anesthesia group (24± 6) was decreased than control (29±7) and short term anesthesia group(30±6)(F=3.29,P＜0.05) with no significant difference in correct rate at 28 d after anesthesia.There was no difference in latent time of water maze between groups at age of 4 month.The ratios of spent time in the target quadrant to the whole time at age of 4 month at 11 d and 28 d after anesthesia in short-term anesthesia group[( 36.6 ± 14.4)％,(34.7 ±9.5)％] and long term anesthesia group [( 36.8 ± 16.4)％ ,(31.8± 12.0)％] were reduced as compared with control [(49.5±8.8) ％,(42.8±12.2) ％] (F=3.31,3.30,all P＜0.05).The latent time of mice at age of 20 month at 11 d after anesthesia in short-term anesthesia[(31±6)s] and longterm anesthesia group [(30±7)s ] were longer than control [(23±6)s](F =3.34,P＜0.05).There were no differences in the ratios of spent time in the target quadrant to the whole time and the number of cross -platform among the groups. Conclusions Isoflurane anesthesia may impact the mediumand long- term cognitive funclion in mice at ages of 4 month and 20 month.
