Bone Formation By Human Alveolar Bone Cells.
- Author:
Byung Ho CHOI
1
;
Jin Hyoung PARK
;
Jin Young HUH
;
Jin Rok OH
Author Information
1. Department of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Tissue engineering;
Cell culture;
Alveolar bone
- MeSH:
Animals;
Cell Culture Techniques;
Humans*;
Mice;
Osteocytes;
Osteogenesis*;
Tissue Engineering;
Tooth Extraction;
Transplants
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2002;28(1):42-45
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Cultures of primary human alveolar bone-derived cells were established from alveolar bone chips obtained from normal individuals undergoing tooth extraction. These cells were expanded in vitro until passage 3 and used for the in vivo assays. Cells were loaded into transplantation vehicles, and transplanted subcutaneously into immunodeficient mice to study the capacities of human alveolar bone-derived cells to form bone in vivo. Transplants were harvested 12 weeks after transplantation and evaluated histologically. Of 10 human alveolar bone-derived cell transplants, two formed a bone-like tissue that featured osteocytes and mineral. Eight of the ten formed no osseous tissue. These results show that cells from normal human alveolar bone are capable of forming bone-like tissue when transplanted into immunodeficient mice.
