Expression of neuronal intermediate filament protein (NF-66) and relationship with benign and malignancy of insulinomas
10.3760/cma.j.issn.1674-1935.2012.02.005
- VernacularTitle:神经中间丝蛋白NF-66与胰岛素瘤良恶性的关系
- Author:
Bei LIU
;
Yu XIAO
;
Liming ZHU
;
Xinwei QIAO
;
Hongding XIANG
;
Jie CHEN
;
Yuanjia CHEN
- Publication Type:Journal Article
- Keywords:
Insulinoma;
Intermediate filament proteins;
Diagnosis,differential;
Prognosis
- From:
Chinese Journal of Pancreatology
2012;12(2):89-91
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether neuronal intermediate filament protein(NF-66) could be used as a molecular marker for the determination of malignancy of insulinoma.MethodsThe expression of NF-66 protein was detected in insulinoma and pana - cancerous tissues and 3 insulinoma cell lines by immunohistochemistry staining. The relationship between the expression of NF-66 protein and the clinicopathological characteristics,survival was analyzed by univariate and multivariate statistical analysis.ResultsExpression of NF-66 was found in 102(77％ ) out of 132 insulinomas and none of 98 paired control (P =4.86 × 10-31 ).NF-66 was highly expressed in 3 insulinoma cell lines.The expression of NF-66 was found in 96 (81％) out of 118 benign tumors (P =0.003),while out of 14 malignant insulinomas,6(43％ ) were found to express NF-66 ( P =0.003 ).The expression of NF-66 was significantly associated with tumor size (3 cm as the cut-off point),distant metastasis (38％ vs 81％,P =0.013) and distant plus lymph node metastasis (46％ vs 81％,P =0.009),respectively.The expression of NF-66 was not correlated with age,gender,recurrence and overall survival.ConclusionsDown-regulation of NF-66 was significantly associated with tumor malignancy,suggesting that NF-66 could be a potentially novel molecular bionarker to distinguish malignancy from benign insulinoma.
