Effects of MMI-166 on proliferation and apoptosis in human pancreatic cancer SW1990 cell
10.3760/cma.j.issn.1674-1935.2012.02.008
- VernacularTitle:MMI-166对人胰腺癌细胞株SW1990体外增殖及凋亡的影响
- Author:
Bengang GONG
;
Huaiyong XU
;
Piguang CHENG
;
Chongchong GAO
;
Junben WU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Cell line,tumor;
Tissue inhibitor of metalloproteinases;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Pancreatology
2012;12(2):100-102
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of MMI-166 on the proliferation and apoptosis of human pancreatic cancer SW1990 cells.MethodsMMI-166 of different concentrations (25,50,100 μg/ml) were used to treat human pancreatic cancer SW1990 cell for 24,48 h.Effect of MMI-166 on cell proliferation wasdetected by 3- (4,5-dimethyl-2-thiazole) -2-5-biphenly-tetrazole bromide ( MTT ) method and effect on cell apoptosis was tested by Annexin V-PI method and flow cytometry (FCM).ResultsTwenty-four hours after MMI-166 treatment of different concentrations (25,50,100 μg/ml),the inhibitory rates of the cells were (34.23±3.87)％,(44.81 ±2.01)％,(53.91 ±1.74)％,and the corresponding values were (39.95 ± 1.83) ％,( 52.26 ± 3.46 ) ％,( 63.20 ± 2.48 ) ％ at 48 h,which suggested a time-and concentrationdependent manner.The cell's apoptosis rates were (11.19 ±0.47)％,(23.01 ±0.53)％,(28.10 ± 0.52) ％ at 24 h,and the corresponding values were ( 11.19 ± 0.47 ) ％,( 23.01 ± 0.53 ) ％,( 28.10 ± 0.52)％ at 48 h,which were significantly higher than those in control group [ (0.09 ±0.12)％,P ＜0.05].ConclusionsMMI-166 can inhibit proliferation and induce apoptosis of human pancreatic SW1990 cell in a time- and concentration-dependent manner.
