Effects of PTEN and KAI1 double gene transfection on proliferation, metastasis on pancreatic cancer AsPC1 cells under hypoxic condition
10.3760/cma.j.issn.1674-1935.2012.02.014
- VernacularTitle:乏氧条件下PTEN、KAI1基因双转染对胰腺癌AsPC1细胞增殖、转移的影响
- Author:
Hongyu LI
;
Jianjun LI
;
Xiaozhorg GUO
;
Xu LIU
;
Chunyan WU
;
Jiajun ZHAO
;
Yanzhi CHEN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Cell line,tumor;
Anoxia;
Transfection
- From:
Chinese Journal of Pancreatology
2012;12(2):120-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of PTEN and KAI1 double gene transfection on proliferation,metastasis in AsPC1 pancreatic cancer cells under hypoxic condition.MethodsRecombinant vectors that over expressing PTEN and KAI1 protein which was established previously were double transfected into hypoxic AsPC1 cells.Western blot was performed to measure the expression level of PTEN and KAI1.Then,cell proliferation was detected by MTT,and colony forming assay were used to test the ability of tumor cells forming colonies,and transwell assay was used to evaluate metastatic function.ResultsAfter double gene trnsfection,both PTEN and KAI1 protein expression was significantly up-regulated,which was 2.05 and 1.5 1 folds higher than that of empty vector group; and cell proliferation of hypoxic AsPC1 cells was suppressed significantly (0.5 vs 0.8,P =0.00031 ),number of colony formation was significantly decreased [ (41.67 ± 5.03) vs (86.00 ± 7.81 ),P =0.017) ] ; the capacity of metastasis was significantly decreased (0.68 ± 0.05 vs 1.23 ± 0.03,P =0.0025 ).ConclusionsDouble gene transfection of PTEN and KAI1 could inhibit proliferation and metastatic activity of hypoxic AsPC1 cells,which might indicate that combined gene therapy may play a role in the treatment of pancreatic cancer.
