Effects of simvastatin preconditioning on inducible and endothelial nitric oxide synthase expression in thoracic aorta in a rat model of sepsis
10.3760/cma.j.issn.0254-1416.2012.02.031
- VernacularTitle:辛伐他汀预处理对脓毒症大鼠胸主动脉诱导型一氧化氮合酶及内皮型一氧化氮合酶表达的影响
- Author:
Minzhi LI
;
Donglian TIAN
;
Min LI
;
Aihong WANG
;
Limin LI
;
Long ZHENG
;
Heling ZHAO
- Publication Type:Journal Article
- Keywords:
Lovastatin;
Sepsis;
Nitric oxide synthase type Ⅱ;
Nitric oxide synthase type Ⅲ;
Aorta,thoracic
- From:
Chinese Journal of Anesthesiology
2012;32(2):243-246
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of simvastatin preconditioning on the expression of inducible and endothelial nitric oxide synthase ( iNOS,eNOS) in thoracic aorta in a rat model of sepsis.Methods Eighty pathogen-free female Wistar rats aged 4 months weighing 200-250 g were randomly divided into 4 groups:group normal control (group Ⅰ,n =8) ; group sham operation (group Ⅱ,n =8) ; group sepsis (group Ⅲ,n =32) and group simvastatin preconditioning (group Ⅳ,n =32).Sepsis was induced by cecal ligation and puncture (CLP) in groups Ⅲ and Ⅳ.In group Ⅳ simvastatin 20 mg/kg was given via a gastric tube once a day for 2 weeksbefore CLP.The thoracic aorta specimens were taken at 3,6,24 and 48 h after CLP (n =8 at each time point)for detection of iNOS and eNOS protein expression by Western blot analysis.ResultsCLP significantly up-regulated iNOS expression and down-regulated eNOS expression in group Ⅲ as compared with groups Ⅰ and Ⅱ.Simvastatin pretreatment significantly attenuated CLP-induced increase in iNOS expression and decrease in eNOS expression in group Ⅳ as compared with group Ⅲ.ConclusionSimvastatin preconditioning can protect vascular endothelial cells from septic injury by down-regulating iNOS expression and up-regulating eNOS expression in vascular endothelial cells.
