Differentiation into oligodendrocytes of bone marrow stromal cells transplanted to the injured spinal cord
10.3760/cma.j.issn.1671-7600.2012.09.014
- VernacularTitle:骨髓基质干细胞在损伤脊髓内向少突胶质细胞定向分化的研究
- Author:
Shaoqiang CHEN
;
Jianhua LIN
- Publication Type:Journal Article
- Keywords:
Bone marrow cells;
Cell differentiation;
Immunohistochemistry;
Spinal cord injuries
- From:
Chinese Journal of Orthopaedic Trauma
2012;14(9):795-799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.
