The detection of the binding protein of chlamydiaphage phiCPG1 capsid protein Vp1 on chlamydial outer membrane of serotype D
10.3760/cma.j.issn.1000-6680.2012.10.002
- VernacularTitle:衣原体噬菌体phiCPG1衣壳蛋白Vp1在D型沙眼衣原体外膜中结合位点的检测
- Author:
Yuanjun LIU
;
Yina SUN
;
Weifeng YAO
;
Yan LI
;
Zhuoran LI
;
Jiurong WEI
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Bacteriophages;
Capsid;
Bacterial outer membrane proteins
- From:
Chinese Journal of Infectious Diseases
2012;30(10):583-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the binding protein of chlamydiaphage phiCPG1 capsid protein Vp1 on chlamydia trachomatis outer membrane.Methods The bacterium with recombinant plasmid Vp1/pet30a( + ) was induced.The expressed protein was purified by gel recycling.FarWestern blot was utilized to' investigate the binding protein of Vp1 on chlamydial outer membrane,including recombinant polymorphic outer membrane protein (rPmp) and major outer membrane protein (MOMP).Results The recombinant protein Vp1 was successfully expressed in E.coli.Monoclonal antibody against Vp1 was used as primary antibody in Western blot,and no specific band was present,which indicated that the monoclonal antibody did not specifically bind with any rPmp.Far-Western blot results showed that there was an obvious band for the rPmpI,but no specific band for other rPmp and MOMP,which suggested that Vp1 could specifically bind with rPmpI protein on the chlamydial outer membrane of serotype D.Conclusions There is a binding site of Vp1 on the chlamydia trachomatis outer membrane.Vp1 may play an important role in the interaction between the chlamydiaphage and the chlamydiae.
