Effects of ozone oxidative preconditioning on apoptosis induced by renal ischemia/reperfusion injury in rats
10.3760/cma.j.issn.0254-1785.2012.02.012
- VernacularTitle:臭氧氧化预处理对大鼠肾脏缺血再灌注损伤致细胞凋亡的影响
- Author:
Jiangqiao ZHOU
;
Hui CHEN
;
Zhiyuan CHEN
;
Minghuan GE
;
Hengcheng ZHU
;
Yunfei HU
;
Xiuheng LIU
- Publication Type:Journal Article
- Keywords:
Rats;
Kidney;
Ozone;
Oxidative preconditioning;
Reperfusion injury;
Apoptosis
- From:
Chinese Journal of Organ Transplantation
2012;33(2):113-117
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the protective effect of ozone oxidative preconditioning (OzoneOP) on apoptosis induced by acute renal ischemia/reperfusion (I/R) injury in rats.Methods The right kidneys of rats in control group were excised.A rat I/R model was established in I/R group.In OzoneOP group,the renal OzoneOP was induced by rectal insufflation of 5.0～5.5 ml oxygen plus ozone (ozone 50 mg/L,1 mg· kg- 1 · d- 1,once every day).The parameters (blood urea nitrogen and creatine )of renal function were determined by auto-biochemical analyzer. The concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured by using chromometry.Cytochrome c (CytC) was examined by using immunohistochemistry and Western blotting.The mRNA expression levels of TNF-α,IL-1β and IL-6 in the renal tissue were detected by using RT-PCR.ResultsCompared with the control group,the blood urea nitrogen,creatine,MDA and the release of CytC were increased significantly in I/R group.After OzoneOP,the levels of blood urea nitrogen,creatine,MDA,CytC and the mRNA expression levels of TNF-α,IL-1-β and IL-6were significantly decreased and SOD levels were significantly increased.ConclusionOzoneOP could relieve renal I/R injury of rats by improving antioxidation capability,cutting down cytokines contents and inhibiting the release of CytC from mitochondria.
